pP{Target70} is a derivative of pP{TargetB}. The CaSpeR-derived mini-white gene was replaced by a white cDNA expressed under the basal hsp70 promoter, much like the pW8 vector or P{FRT-whs-FRT} construct.
pP{Target70} Features |
|
1-235 |
3'P end |
296-329 |
FRT |
336-413 |
polylinker |
414-437 |
I-CreI site |
533-714 |
hsp70 promoter |
715-901 |
hsp70 5'UTR |
933-3319 |
white cDNA |
3335-3368 |
FRT |
34058-3979 |
5'P end |
39803-6022 |
pUC backbone (AmpR) |
pP{Target70} Sequence
CATGATGAAATAACATAAGGTGGTCCCGTCGGCAAGAGACATCCACTTAACGTATGCTT
GCAATAAGTGCGAGTGAAAGGAATAGTATTCTGAGTGTCGTATTGAGTCTGAGTGAGAC
AGCGATATGATTGTTGATTAACCCTTAGCATGTCCGTGGGGTTTGAATTAACTCATAAT
ATTAATTAGACGAAATTATTTTTAAAGTTTTATTTTTAATAATTTGCGAGTACGCAAAG
ctagagttgttggttggcacaccacaaatatactgttgccgagcacaattccgctcgac
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCggatccAGATCTGCTAGCGGCCGCA
TGCCGCGGACGCGTAGGCCTGCAGGAATTCGATTCGACCTCGAGGGGGGGCCCGGTACC
ACCAAACTGTCTCACGACGTTTTGcaattctagtatgtatgtaagttaataaaacccat
ttttgcggaaagtagataaaaaaaacattttttttttttactgcactggatccttgccc
cgttattctctattcgttttgtgactctccctctctgtactattgctctctcactctgt
cgcacagtaaacggcactctattctcgttgcttcgagagagcgcgcctcgaatgttcgc
gaaaagagcgccggagtataaatagaggcgcttcgtcgacggagcgtcaattcaattca
aacaagCAAAGTGAACACATCGCGAAGCAAGCTGAGCAAACAAACAAGCGCAGCGAACA
AGCTAAACAATCTGCAATAAAGTGCAAGTTAAAGTGAATCAATTAAAAGTAACCAACAA
CCAAGTAATTAAACTAAAAACTGCAACTACTGAAATCAACCAAGAAGTCATTATTGAAG
ACAAGAAGAGAACTCTgaataggtcgatagcgtcaattcggcacgaggtttcgtgacga
agctccaagcggtttacgccatcaattaaacacaaagtgctgtgccaaaactcc
tctcgcttcttatttttgtttgttttttgagtgattgggtgggtgattggttttgggtgggtaagc
aggggaaagtgtgaaaaatcccggcaATGGGCCAAGAGGATCAGGAGCTATTAATTCGCGGAGGCA
GCAAACACCCATCTGCCGAGCATCTGAACAATGGTGACAGCGGAGCGGCTTCGCAGAGCTGCATTA
ACCAGGGCTTCGGGCAGGCCAAAAACTACGGCACGCTCCTGCCACCCAGTCCGCCGGAGGACTCCG
GTTCAGGGAGCGGCCAACTAGCCGAGAACCTCACCTATGCCTGGCACAATATGGACATCTTTGGGG
CGGTCAATCAGCCGGGCTCCGGATGGCGGCAGCTGGTCAACCGGACACGCGGACTATTCTGCAACG
AGCGACACATACCGGCGCCCAGGAAACATTTGCTCAAGAACGTTTGCGGCGTGGCCTATCCGGGCG
AACTTTTGGCCGTGATGGGCAGTTCCGGTGCCGGAAAGACGACCCTGCTGAATGCCCTTGCCTTTC
GATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATGCGACTGCTCAATGGCCAACCTGTGGACG
CCAAGGAGATGCAGGCCAGGTGCGCCTATGTCCAGCAGGATGACCTCTTTATCGGCTCCCTAACGG
CCAGGGAACACCTGATTTTCCAAGCCATGGTGCGGATGCCACGACATCTGACCTATCGGCAGCGAG
TGGCCCGCGTGGATCAGGTGATCCAGGAGCTTTCGCTCAGCAAATGTCAGCACACGATCATCGGTG
TGCCCGGCAGGGTGAAAGGTCTGTCCGGCGGAGAAAGGAAGCGTCTGGCATTCGCCTCCGAGGCAC
TAACCGATCCGCCGCTTCTGATCTGCGATGAGCCCACCTCCGGACTGGACTCATTTACCGCCCACA
GCGTCGTCCAGGTGCTGAAGAAGCTGTCGCAGAAGGGCAAGACCGTCATCCTGACCATTCATCAGC
CGTCTTCCGAGCTGTTTGAGCTCTTTGACAAGATCCTTCTGATGGCCGAGGGCAGGGTAGCTTTCT
TGGGCACTCCCAGCGAAGCCGTCGACTTCTTTTCCTACGTGGGTGCCCAGTGTCCTACCAACTACA
ATCCGGCGGACTTTTACGTACAGGTGTTGGCCGTTGTGCCCGGACGGGAGATCGAGTCCCGTGATC
GGATCGCCAAGATATGCGACAATTTTGCCATTAGCAAAGTAGCCCGGGATATGGAGCAGTTGTTGG
CCACCAAAAATCTGGAGAAGCCACTGGAGCAGCCGGAGAATGGGTACACCTACAAGGCCACCTGGT
TCATGCAGTTCCGGGCGGTCCTGTGGCGATCCTGGCTGTCGGTGCTCAAGGAACCACTCCTCGTAA
AAGTGCGACTTATTCAGACAACGATGGTTGCCATCTTGATTGGCCTCATCTTTTTGGGCCAACAAC
TCACGCAAGTGGGTGTGATGAATATCAACGGAGCCATCTTCCTCTTCCTGACCAACATGACCTTTC
AAAACGTCTTTGCCACGATAAATGTGTTCACCTCAGAGCTGCCAGTTTTTATGAGGGAGGCCCGAA
GTCGACTTTATCGCTGTGACACATACTTTCTGGGCAAAACGATTGCCGAATTGCCGCTTTTTCTCA
CAGTGCCACTGGTCTTCACGGCGATTGCCTATCCGATGATCGGACTGCGGGCCGGAGTGCTGCACT
TCTTCAACTGCCTGGCGCTGGTCACTCTGGTGGCCAATGTGTCAACGTCCTTCGGATATCTAATAT
CCTGCGCCAGCTCCTCGACCTCGATGGCGCTGTCTGTGGGTCCGCCGGTTATCATACCATTCCTGC
TCTTTGGCGGCTTCTTCTTGAACTCGGGCTCGGTGCCAGTATACCTCAAATGGTTGTCGTACCTCT
CATGGTTCCGTTACGCCAACGAGGGTCTGCTGATTAACCAATGGGCGGACGTGGAGCCGGGCGAAA
TTAGCTGCACATCGTCGAACACCACGTGCCCCAGTTCGGGCAAGGTCATCCTGGAGACGCTTAACT
TCTCCGCCGCCGATCTGCCGCTGGACTACGTGGGTCTGGCCATTCTCATCGTGAGCTTCCGGGTGC
TCGCATATCTGGCTCTAAGACTTCGGGCCCGACGCAAGGAGTAGccgacatatatccgaaataact
gcttgttttttttttttaccattattaccatcgtgtttactgtttattgccccctcaaaaagctaa
tgtaattatatttgtgccaataaaaacaagatatgacctatagaatacaaaaaaaaaaaaaaaaaa
aaaaaaaaaaactcgatcgagtcgacGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCggatccaaacctagtaatta
attcggctgctgctctAAACGACGCATTTCGTACTCCAAAGTACGAATTTTTTCCCTCA
AGCTCTTATTTTCATTAAACAATGAACAGGACCTAACGCACAGTCACGTTATTGTTTAC
ATAAATGATTTTTTTTACTATTCAAACTTACTCTGTTTGTGTACTCCCACTGGTATAGC
CTTCTTTTATCTTTTCTGGTTCAGGCTCTATCACTTTACTAGGTACGGCATCTGCGTTG
AGTCGCCTCCTTTTAAATGTCTGACCTTTTGCAGGTGCAGCCTTCCACTGCGAATCATT
AAAGTGGGTATCACAAATTTGGGAGTTTTCACCAAGGCTGCACCCAAGGCTCTGCTCCC
ACAATTTTCTCTTAATAGCACACTTCGGCACGTGAATTAATTTTACTCCAGTCACAGCT
TTGCAGCAAAATTTGCAATATTTCATTTTTTTTTATTCCACGTAAGGGTTAATGTTTTC
AAAAAAAAATTCGTCCGCACACAACCTTTCCTCTCAACAAGCAAACGTGCACTGAATTT
AAGTGTATACTTCGGTAAGCTTCGGCTTTCGACGGGACCACCTTATGTTATTTCATCAT
Gggccagacccacgtagtccagcggcagatcggcggcggagaagttaagcgtctccagg
atgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagctaatttcgcccgg
ctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacggaaccatgaga
ggtacgacaaccatttgaggtatactggcaccgagcccgagttcaagaagaaggcgttt
ttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg
gcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgc
gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggga
agcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcg
ctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccg
gtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcc
actggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtg
gtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagc
cagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggt
agcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaaga
agatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaag
ggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaa
tgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatg
cttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct
gactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgct
gcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcc
agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta
ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgtt
gttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcag
ctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcgg
ttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactc
atggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttc
tgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagtt
gctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtg
ctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgag
atccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttca
ccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagg
gcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcattta
tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaa
taggggttccgcgcacatttccccgaaaagtgccacctgcgaagtcttagagccagata
tgcgagcacccggaagctcacgatgagaatggccagac
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